Preparation of 3-bromo-2,4,6-trimethyl HIDA and its 99m Tc-complex for hepatobiliary imaging
Abstract
3-bromo-(2,4,6-trimethylphenylcarbamoylmethyl) iminodiacetic acid (3-bromo-2,4,6-trimethyl HIDA) was synthesized, and lyophilized vials were prepared which contained 20 mg of 3-bromo-2,4,6-trimethyl HIDA and 0.4 mg of SnCl 2 . Radiochemical and biologic studies showed that this agent was obtained in high radiochemical purity, were stable in vitro and in vivo conditions. The compound, 99m Tc-3-bromo-2,4,6-trimethyl HIDA, possessed high hepatic specificity meaning that none of the tissues except for the hepatobiliary system showed radioactivity concentrations, and rapid hepatocellular transit and a rapid clearance from the blood circulation was observed. In conclusion, a lyophilized kit of HIDA and its labeling with 99m Tc can be applied as a hepatobiliary imaging agent for the evaluation of the functional status of the hepatocytes and the patency of the biliary duct.