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Chitosan-silica nanocomposite sorbent for thin-layer chromatography of alkaloids

B. D. Kabulov“Fan va Tarakkiet” Scientific and Technical Center, Tashkent State Technical University, Tashkent, UzbekistanD. Sh. Shakarova“Fan va Tarakkiet” Scientific and Technical Center, Tashkent State Technical University, Tashkent, UzbekistanО. А. ШпигунFaculty of Chemistry, Moscow State University, Leninskie Gory, Moscow, 119992, RussiaS. S. Negmatov“Fan va Tarakkiet” Scientific and Technical Center, Tashkent State Technical University, Tashkent, Uzbekistan
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Abstract

The feasibility of using a chitosan-silica nanocomposite sorbent in thin-layer chromatography of cytisine alkaloid and some of its derivatives was studied. The derivatives were obtained by the reactions of cytisine with aromatic aldehydes containing the -OH, -OCH3, and -Br functional groups as substituents in different benzene ring positions. The separation of cytisine and its derivatives on the chitosan-silica sorbent was more effective than on initial silica gel. The mechanism of chromatographing on the two sorbents was considered; the mobile phase was a 6:1 (v/v) chloroform:methanol mixture.

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