Effects of Several Factors on Adventitious Bud Induction of Eucalyptus dunnii
Abstract
Experiments were conducted to study the effect of different explants,light source,light intensity and antioxidant on adventitious bud induction of Eucalyptus dunnii.The experimental results indicated that the bud germination ratio of sprouting branches was higher than that of crown new shoot.Natural illumination was good for the subsequent generation,the light of 800~1 000 lux(7~10d),then change to light of 2 500~3 000 lux was suitable for the growth of adventitious bud.Vc(10 ~15 mg/L) and VB2(15~20 mg/L) were helpful for slowing or restraining buds from getting brown.Adventitious buds from different superior plants exhibited differently in growth and multiplication,the multiplication rate of GLD1,GLD2,GLD3,GLD4 and GLD6 was 2.8 ~ 3.4 times in 25d.