The Establishment of Xanthoceras sorbifolia Bunge Somatic Embryogenesis Eystem
Abstract
In this paper,used young fruit period,mid period and maturity of Xanthoceras sorbifolia Bunge seed embryos as explants.Using tissue culture techniques, screened the factors that affecting Xanthoceras sorbifolia Bunge somatic embryogenesis.Established a stable training system for Xanthoceras sorbifolia Bunge somatic embryogenesis.The results showed that it was better to use mid period of Xanthoceras sorbifolia Bunge seed embryos for inducing somatic cells.Seed embryos formed callus in the dark culture conditions in MS+BA 1.0 mg/L+2,4-D 1.0 mg/L+2% sucrose solid medium.Seed embryos produced somatic embryos in the dark culture.In the MS+BA 2.0 mg/L+NAA 0.05 mg/L+2% sucrose solid medium.The study of somatic embryogenesis for Xanthoceras sorbifolia Bunge lay the groundwork for such studies these were fine clones of propagation,artificial seed production and genetic transformation.