Effects of total phenolics from abnormal savda munziq combined with cisplation,ocetaxd on HeLa cells proliferation and apoptosis
Abstract
Objective To investigate the effects of total phenolics from abnormal savda munziq combined with cisplatin or docetaxel on HeLa cell line of cervical cancer cultured in vitro.Methods HeLa cells were treated with different drug concentrations: total phenolics combined with cisplatin and total phenolics combined with docetaxel,we chose MTT assay to test the inhibiting effects and Flow cytometry to examine cell apoptosis rate.Microscope was used to observe the growth of HeLa cells.Results The inhibiting effect and apoptosis rate of total phenolics combined with cisplatin was greater than cisplatin(P0.05),this phenomenon also appeared in total phenolics combined with docetaxel.Conclusion Total phenolics from abnormal savda munziq combined with cisplatin or docetaxel can inhibit the growth of HeLa cells obviously,and induce apoptosis in vitro,the combinations have synergistic actions.