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Evaluation of the Speed-oligo Mycobacteria assay for the rapid differentiation of frequent Mycobacterium ssp - a multi-centre and multi-country survey

Sabine Hofmann-ThielIML red Gauting, synlab Bayern, Asklepios-FachklinikenL TurayevTashkentTarig M.S. AlnourLore DrathIngelheimM MüllerováHarald HoffmannIML red Gauting, synlab Bayern, Asklepios-Fachkliniken
Pneumologiejournal2011en
ABI

Abstract

A new DNA line probe assay (Speed-oligo Mycobacteria, Vircell) was evaluated for the differentiation of mycobacterial species. It is based on PCR targeting the 16S-23S rRNA internal transcribed spacer region followed by hybridization to specific oligonucleotides by means of a simple dip stick technique.

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