Key Technology of Rapid Propagation in Vitro of Daxing'anling Wild Blueberry

Abstract

The stem segments of wild blueberry were used as the explants to study the best sterilizing method of wild blueberry in vitro culture,the optimal proliferation and rooting culture medium,and culture condition.The results showed that the best sterilizing method of wild blueberry in witro rapid propagation was 0.1% mercuric chloride soaked for 3min;the optim um culture medium was using WPM(improved) + ZT 0.5mg/L that the differentiation rate can be as high as 90%;the optimal rooting medium was 1 /2 WPM(improved) + IBA 1mg / L.The inoculated stem segments cultured in darkness for 20d,and then cultivated wild blueberry seedling in the light.The rooting rate can be as high as 65%.

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