Mutations in the genes of drug-resistant mycobacterium tuberculosis in Uzbekistan
Abstract
Aim of research to study the prevalence of drug resistance in Mycobacterium tuberculosis circulating in various regions of Uzbekistan. Materials and methods: In 2013 were tested 1438 patterns for drug susceptibility. The sensitivity of the MBT to anti TB drugs was determined using a diagnostic kit GenoType® MTBDR plus . Results and discussion: Among the examined patient 793 (55%) were men, women were 645 (45%). By the instrumentality of a diagnostic kit GenoType® MTBDR plus in 40.4% (580) of patients identified sensitive strains of MBT to isoniazid (INH) and rifampicin (RIF). Mutations in the rpoB, katG, inhA genes were found in 59.6% (858) of patients. Mono-resistance to INH was detected at 13% (188) cases. Mono-resistance to RIF was founded at 2.6% (37) patients. In 44% (633) cases identified multi drug resistance (MDR) strains of the MBT, at the same time resistant to RIF and INH. Regional distribution of drug resistance strains: 1.Mono resistance to INH: Tashkent region 30.3% (57 out of 188),Tashkent city 17.5%, Samarqand region 9.5% 2.Mono resistance to RIF: Fergana region 24.4% (9 out of37), Tashkent region 16.2%, Namangan region 13.5% 3.MDR strains: Fergana region 20.9% (132 out of 633), Tashkent region 18.3%, Andijan region 17%, Tashkent city 19.3% Conclusion: Thus, in the structure of drug resistance strains dominated MDR strains (44%). The largest number of cases with mono-resistance to RIF occurred in the Fergana region (24.4%) and mono-resistance to INH in the Tashkent region (30.3%). MDR strains of MBT were more common at Fergana (20.9%) and Andijan (17%) regions. Further inquiry of the problem continues.