Rapid Diagnostics of a Human Cervical Cancer Cells Under Reflected Light Microscope
Abstract
A technique for revealing surface morphology of human cervical cancer cells has been developed to facilitate early diagnostics of a pre-cancer and cancer cells under reflected light microscopy. The offered method was borrowed from optical microscopy of a solid state surface where the metallographic inverted microscopy (MIM) are usually used. Unlike common accepted transmitted light microscopy for biological applications MIM technique allows to reveal a morphology and topology of a biological cells surface without any treatment by chemicals (fixing, staining, drying, freezing et al). The MIM method was demonstrated by analyzing fresh native smears from epithelium of uterine neck. MIM micrographs of 167 patients with diagnosis cervical cancer allow to visualize on the cancer cells surface numerous of the light reflective formations (LRF). It is supposed that LRF are connected with exocytosis on the cell membrane.