Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
С. А. СасмаковAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanOybek N. AshirovAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanJaloliddin AbdurakhmanovAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanShukhrat Sh. KhasanovAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFarkhod EshboevAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanElvira YusupovaAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanG. A. PiyakinaAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanShakhnoza S. AzimovaAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, Uzbekistan
ABI
Abstract
"The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp) the recombinant strains of P. pastoris producing the PreS2-S region of surface antigen of HBV were obtained. The PAGE electrophoresis and immunoblotting of obtained recombinant PreS2-S protein confirm the molecular weight (34 kDa) and high specificity to the HBV antibodies."
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