Mechanisms of ischemic liver damage disorders and ways of their correction using a new amino acid mixture based on sodium succinate and mannitol.
Abstract
Abstract. The aim of the study. Conduct an open-label randomized study to study the effectiveness of a new amino acid mixture based on sodium succinate and mannitol in the course of experimental toxic hepatitis. Materials and research methods. Acute heliotrin intoxication was reproduced by a single subcutaneous administration of a sublethal dose of heliotrin to rats, prepared at the rate of 40 mg per 100 g of body weight. Toxic hepatitis was reproduced by subcutaneous administration of heliotrin (25 mg / 100 g). The material for the study is venous blood. Indicators of protein balance were studied: total serum protein, albumin and globulin and biological materials (ALT, AST, bilirubin and alpha-amylase by biochemical analysis with HIF-1 content in the blood was determined by enzyme immunoassay. Animals were divided into equal groups: Group I - before reproduction of heliotrin intoxication (intact), group II (control) - with heliotrin intoxication, group III (control, comparison) - with heliotrin intoxication after the introduction of the reference drug "Infezol 40", within 5 days 24 hours after the last injection; IV group (main, experimental) - animals with heliotrin intoxication after the introduction of a new amino acid blood substitute, within 5 days 24 hours after the last injection Statistical processing was performed using the Student-Fisher test, the nonparametric Mann-Winney test, the Kraskes-Wallis test. Research studies. During the reproduction of the experimental toxic hepatitis by the introduction of heliotrin, it was found that the content of HIF-1 was on average 0.101667 ± 0.0022 ng / l. In blood plasma, the mean HIF-1 values were 0.2136 ± 0.0066 ng / L. After treatment, in group I, HIF-1α values were 0.317 ± 0.022 (p <0.01), in group II - 0.404 ± 0.031 (p≤0.02), in group III - 0.365 ± 0.026 (p≤0.001), in group IV group - 0.421 ± 0.028 (p≤0.001). During the reproduction of experimental toxic hepatitis by the introduction of heliotrin, it was found that the ALT content was on average 25.93 ± 2.91 U / L, and the AST content was at the level of 22.23 ± 1.95 U / L. The de Rits were at 1.17 ± 0.16. Direct bilirubin was at the level of 3.90 ± 0.44 mmol / L, indirect bilirubin - 8.10 ± 0.8 mmol / L. The total bilirubin was 12.01 ± 1.16 mmol / L. Moreover, OR (odds ratio) was 0.93219976. The 95% CI (confidence interval) was 0.88765239. χ 2 = 0.9633286 (Wilconson test). Mann-Winney test (U test) was 0.87219981 at p <0.05.\n\nConclusions: The developed amino acid mixture is superior to traditional methods of treatment (Infezol) in terms of the effectiveness of influence on the development and course of experimental toxic hepatitis, which is proved by the study.