Regulation of Transport of Ca2+ NMDA-Receptors in Rat Brain Synaptosomes Under the Influence of Polyphenols
Abstract
It is known that disturbance of Ca 2+ dynamics in nerve cells is universally involved in all pathologies of Alzheimer's disease (AD). It is likely that the use of chemical agents or small molecules specific for Ca 2+ channels or membranes of intracellular organelles to correct Ca 2+ dysregulation of neurons may open up a new approach to the prevention and treatment of AD. The experiments were carried out according to the Weiler method. Synaptosomes were isolated from the brain of rats by two-stage centrifugation at a temperature of 4C.To measure the amount of cytosolic Ca 2+ synaptosomes were calculated by the Grinkevich equation. The results of the experiments show that there is a possibility of competition between different polyphenols of Caralinia, Granatuum and glutamate for the site of regulation of the opening ion channels f NMDA-receptors.