ASSESSMENT OF CARDIOPROTECTIVE PROPERTIES CONJUGATE DKV-11
Abstract
Abstract: This study determined the cardioprotective property of the conjugate (3,4-dihydroxyphenyl) -6- [1- (2´-bromo-4´, 5´-dimethoxyphenyl) -6,7-dimethoxy-3,4-dihydroisoquinoline-2 (1n) -yl] methyl-3,5,7-trihydroxychroman-4-oн (DKV-11) under conditions of hypoxia and oxidative stress on the activity of contraction of the papillary muscles of the rat heart. The contractile activity of papillary muscle preparations was studied in vitro using SI-BAM21-LC (World Precision Instruments Inc. (WPI); USA) on a device that registers the force of muscle contraction using mechanography. When evaluating the cardioprotective properties of the DKV-11 conjugate in an in vitro model of hypoxia caused by the replacement of 95% O2 and 5% CO2 in an incubation medium with 95% N2 and 5% O2 and in the presence of H2O2 (100 μM) under conditions of hypoxia and oxidative stress. The study showed that the DKV-11 conjugate had an effective cardioprotective property based on a positive inotropic effect on the contractile activity of the papillary muscles of the rat heart.