CHARACTERISTICS OF THE DISTRIBUTION OF ALLELIC POLYMORPHISM OF PRO-INFLAMMATORY CYTOKINE GENES (TNF-α (G308A), IL2 (T330G), IL6 (C174G), IL10 (C592A)) IN PURULENT-INFLAMMATORY DISEASES OF THE MIDDLE EA
Abstract
<strong>Objective: </strong>Study of the distribution of single nucleotide polymorphisms of the genes of pro- inflammatory cytokines involved in the initiation of inflammation - TNF-α (G308A), IL2 (T330G), IL6 (C174G) and IL10 (C592A) among patients with pyoinflammatory diseases of the middle ear <strong>Methods: </strong>The material for the study of pus released from the middle ear was performed in all patients with a diagnosis of CHSO who were admitted to the hospital for treatment. All genotypic variants of the studied genetic polymorphisms (TNF-α (G308A), IL2 (T330G), IL6 (C174G), IL10 (C592A) in the studied groups were analyzed by Hardy-Weinberg equilibrium (RHB, p>0.05). DNA extraction was carried out using the AmpliPrim RIBO-prep commercial kit (JSC Interlabservis, Russia). The OpenEpi 2009, version 2.3 software package was used as a tool for calculating the results of DNA extraction. DNA extraction was carried out using the AmpliPrim RIBO-prep commercial kit (JSC Interlabservis, Russia). According to the instructions, 30 mkl of the required sorbent was added to a special laboratory container (test tube) with 1–2 ml of biomaterial. The resulting mixture was stirred in a vortex mixer and left at a temperature of 600°C for 5 minutes. Molecular markers were detected on a Rotor Gene Q device (Quagen, Germany) by allele-specific PCR in Real-Time format using the NPF Litex assembly (Russia). <strong>Results: </strong>In the general group of patients (Group I), the observed frequencies of the G/G and G/A genotype variants were 0.87 and 0.13, respectively, depending on the distribution of the proportion of the TNF-α single nucleotide polymorph. (G308A) genotypes. Their unexpected frequencies were 0.88 and 0.12, respectively. At the same time, the frequencies of the A/A mutant genotype are zero. At the same time, it should be noted that the difference between the frequencies of Ho and He of the G/G and G/A genotypes is not statistically significant (χ2=0.4; P=0.506; df=1), which means that in the canonical distribution of RXB (p>0 .05) showed no difference. A similar statistical analysis performed among patients with mild purulent-inflammatory diseases of the middle ear (group II) made it possible to determine the frequency distribution of the genotypes of the TNF-α (G308A) single nucleotide polymorphism without deviations from RCM (p>0.05). <strong>Conclusions: </strong>Single nucleotide polymorphism of the TNF-α gene (G308A) is not associated with the occurrence and severity of pyoinflammatory diseases of the middle ear in Uzbekistan. Compared with polymorphic loci of single nucleotide polymorphism IL2 (T330G), it was found that the small allele G (χ2=3.7; P=0.1) increases the likelihood of moderate and severe pyoinflammatory diseases of the middle ear in Uzbekistan. It has been established that the relatively polymorphic polymorphism of the single nucleotide locus of the IL2 gene (T330G) and the small allele G (χ2=3.7; P=0.1) contribute to an increase in moderate and severe pyoinflammatory diseases of the middle ear in Uzbekistan.