Article

Whole genome sequencing of Castanea mollissima and molecular mechanisms of sugar and starch synthesis

Rongchen LiCollege of Biological Sciences and Biotechnology, National Engineering Research Center for Forest Tree Breeding and Ecological Remediation, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing, ChinaXiaolu HuangGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, ChinaLiping YangGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, ChinaJianming LiaoGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, ChinaXiaojuan WeiGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, ChinaJunji LiGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, ChinaGuangyu ZengGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, ChinaDan LiuZhuogong ShiResearch Center for Plateau Characteristic Agriculture in Northeast Yunnan, College of Agriculture and Life Sciences, Zhaotong University, Zhaotong, ChinaZhiheng ZhaoGuangxi Forestry Research Institute, Guangxi Forestry Research Institute, Guangxi Forestry Laboratory, Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization, Nanning, China

Frontiers in Plant Sciencejournal2024en

ABI

Abstract

The chestnut tree exhibits self-incompatibility, where the selection of the male parent (pollen xenia) significantly affects seed starch metabolism, as well as fruit yield and quality. Despite its importance, the molecular mechanisms underlying pollen xenia remains largely unknown. In this study, we utilized the ‘Lan You’ variety of C. mollissima to construct a high-quality reference genome. As a result, a first Telomere-to-telomere (T2T) gap-free genome for this species was successfully assembled. A total of 560 transcription factors and 22 structural genes were identified as consistent across the TO-GCNs, indicating a consistent regulation pattern in the co-expression of genes involved in starch accumulation. These networks were further divided into three sub-networks: T1, T2, and T3. Among these, the T1 and T2 sub-networks exhibited a higher number of structural genes with consistent regulation patterns and were closely associated with sugar biosynthesis. The gene SBE ( Camol08G0254600 ) was identified as the hub gene with the highest degree of connectivity, encoding a key rate-limiting enzyme in the amylopectin biosynthesis pathway. This study provides a foundation for further research on C. mollissima population genetics, genetic improvement, and strategies aimed at enhancing yield and quality.

Topics

Plant Disease Resistance and Genetics Genomics and Phylogenetic Studies Plant Pathogens and Fungal Diseases

Identifiers

DOI: 10.3389/fpls.2024.1455885

Citations and references

Cited by 1 63 references

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