Exclusion liquid chromatography of carboxymethylchitin and carboxymethylchitosan from crustacean <i>Artemia Parthenogenetica</i> of the Aral Sea
Azamat BoymirzaevNamangan Institute of Engineering and TechnologyG. E. BerdimbetovaKarakalpak Research Institute of Natural Sciences, Karakalpak Branch of the Academy of Sciences of the Republic of UzbekistanB. P. KarlybaevaKarakalpak Research Institute of Natural Sciences, Karakalpak Branch of the Academy of Sciences of the Republic of Uzbekistan
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Abstract
The article deals with the synthesis of water-soluble derivatives of chitin and chitosan from cysts of crustaceans of the genus Artemia Parthenogenetica of the Aral Sea by carboxymethylation of OH and NH 2 groups of their chains. The elution, viscosity and polyelectrolyte properties in size exclusion liquid chromatography of carboxymethylchitin and carboxymethylchitosan were studied. The suppression of electrostatic effects and the implementation of the exclusion mechanism for the separation of these polysaccharides were achieved by using an aqueous solution of sodium nitrate with a concentration of 0.1 mol/l as an eluent, which was recommended for determining molecular weight characteristics.
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