Dependence of the reproduction coefficient on donor cells in in vitro improvement of potato cultivars
Abstract
In the experiment, along with growing potato varieties from the meristem cells of the tuber growth, we germinate the botanical seeds of the varieties, grow them from the growth, grow the primary donor cells, obtain healthy callus tissue, grow morphogenetically identical cells, the yield of healthy plant sprouts from the callus tissue is 20% compared to the usual experimental options. 25-30% of callus tissue sprouts, which did not meet the requirements and changed in morphogenesis, remained underdeveloped when callus tissue was grown by taking cells from a normal tumor. It was found that the morphogenesis of the callus sprouts, which were germinated from the seed, obtained from the growths and formed, the weight of the remaining sprouts was 9-10%, and the reproduction coefficient of the sprouts was more than 20%.