ROLE OF THR174MET POLYMORPHISM IN THE AGT GENE IN THE DEVELOPMENT OF PREECLAMPSIA
Abstract
Objective: The causes of preeclampsia are multifactorial, complex and not fully understood. Many scientists believe that the main reason for the development of preeclampsia is profound pathological changes in the vascular system and internal organs of a pregnant woman. The purpose of the study was to investigate the role of the Thr174Met polymorphism in the AGT gene in the development of preeclampsia. Design and method: The main group included 65 pregnant women with severe preeclampsia, the control group included 60 apparently healthy pregnant women. The age of the women examined ranged from 19 to 41 years. The material for the study was DNA samples from patients. Isolation of DNA from blood and PCR analysis were carried out using sets of reagents and test systems from Amply Prime Ribo-prep. Results: A study of the genotypes of the Thr174Met polymorphism in the AGT gene demonstrated that the Thr/Thr genotype, as well as the Thr allele, was insignificantly less likely to be identified among patients with severe preeclampsia (x2=1.92; p=0.17; RR=1.19; 95%CI:0.73-1.94; OR=1.69; 95%CI:0.80-3.56). The Thr/Met genotype, on the contrary, statistically significantly prevailed among this category of patients with severe preeclampsia (x2=1.48; p=0.23; RR=0.72; 95%CI:0.72; OR=0.62; 95%CI:0.29-1.34) The Met/Met genotype also statistically prevailed in severe preeclampsia, relative to patients in the control group (x2=0.29; p=0.61; RR=0.62; 95%CI:0.15-2.56; OR=0.61; 95%CI:0.1-3.71). The heterozygous An increase in the number of unfavorable Thr/Met and Met/Met genotypes of this locus is associated exclusively with an increase in the risk of developing severe preeclampsia. The results of the study indirectly indicate the disregulatory effect of unfavorable genotypic variants of this polymorphism on the expression of angiotensinogen which is an important confirmation of the importance of genetic factors to the formation of arterial hypertension and the development of preeclampsia. Conclusions: According to the odds ratio, functionally unfavorable genotypes can increase this form of pathology up to 5 times. These data allow us to conclude that the Thr174Met polymorphism of the AGT gene makes a certain contribution to the formation of the genetic structure of predisposition to the development and severity of preeclampsia.