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The Role of Stable Anatomical Landmarks in Automated 3D Model Superimposition: A Closer Look

Tommaso CastroflorioClear Aligner Academy, 10100 Torino, ItalySamuele AvoleseDepartment of Mechanical and Aerospace Engineering, Polytechnic University of Turin, 10100 Torino, ItalyFabrizio SannaDepartment of Mechanical and Aerospace Engineering, Polytechnic University of Turin, 10100 Torino, ItalySimone ParriniDepartment of Surgical Sciences, Dental School of the University of Torino, 10100 Torino, Italy
Bioengineeringjournal2025en
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Abstract

OBJECTIVE: To evaluate the concordance of automated 3D superimposition methods applied to digital models, with a focus on methods that consider stable palatal regions as geometric reference landmarks versus those that do not. DESIGN AND SETTING: This was a prospective, cross-sectional study using digital model files of patients undergoing orthodontic treatment in a university clinical setting. PARTICIPANTS: Sixty-one patients were prospectively enrolled and divided into three groups based on the type of orthodontic treatment they received: (20) non-extractive orthodontic treatment without intermaxillary elastics, (21) intermaxillary elastics, and (20) control subjects with no orthodontic movement. The inclusion criteria included the availability of complete pre- and post-treatment digital casts and the absence of significant craniofacial anomalies. METHODS: Three superimposition methods were tested: (1) superimposition according to palate and palatal ridges, (2) best-fit superimposition of arches in occlusion, and (3) best-fit superimposition of individual arches. Discrepancies were identified by comparing the spatial positions derived from each method. Within three spatial axes, deviations of ±0.5 mm and ±1.15° were not considered significant. Bland-Altman plots were used to quantify palatal rugae based and non-based spatial differences between methods. Differences in the superimposition results between the three patient groups were evaluated using ANOVA tests. RESULTS: Differences in spatial position between the superimposition methods often exceeded the acceptable range. The results were compared between the three patient groups with a statistical significance of α = 0.05. In the present study, the high reliability of the superimposition method based on the palate and palatal ridges was observed. CONCLUSION: Superimposition methods based on the palate and palatal rugae provide superior accuracy in determining treatment-related changes in upper arch digital models. These findings illustrate the need for appropriate selection of superimposition techniques based on the study objective of using clinically relevant techniques.

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