The Amount of Flavonoids in Glycyrrhiza glabra L. Plants Artificially Infected with Fusarium velticilliodes in Invitro Conditions
Abstract
In this research, the dynamics of flavonoids were studied by infecting the in vitro plant Glycyrrhiza glabra L with the fungus Fusarium velticilliodes. The amounts of apigenin, gallic acids, kaempferol, quercetin, and rutin in the leaves, stems, and roots of these plants were determined using high-performance liquid chromatography (HPLC). When infected with the fungus, apigenin exhibited antifungal properties in the stems of Glycyrrhiza glabra L, and its amount decreased compared to the control. Gallic acids and rutin, which are considered strong antioxidants, were found to be higher in the leaves than in other parts, and increased to 472.502 mg/L and 933.703 mg/L, respectively, when infected with the fungus. The decrease in the amount of apigenin in the leaves to 14.218 mg/L, in the stems to 107.636 mg/L, and in the roots to 14.133 mg/L after fungal infection indicates the manifestation of the antifungal properties of this flavonoid. The increase in the amount of kaempferol and quercetin in the leaves to 84.088 mg/L and 69.398 mg/L after infection with Glycyrrhiza glabra L, respectively, scientifically confirms that flavonoids are an important part of the antioxidant defense system in plant tissues, especially in response to stress factors, the synthesis of phenolic compounds is activated.