Study of pharmacokinetics of gentamicin in rats under conditions of lymphotropic and intramuscular administration
Abstract
The study of the pharmacokinetics of antibiotics on model objects makes it possible to optimize their dosage and method of administration for further clinical practice. Aim. The aim of this study was to compare the pharmacokinetics of gentamicin sulfate in rats following intramuscular and lymphotropic pretracheal administration. Materials and methods. The disk diffusion method was used to determine the antibiotic concentration in blood and tissue homogenate for Escherichia coli NCTC 8172. Gentamicin sulfate concentration was highest in blood samples after intramuscular administration for the first hour and lowest in pleura. In the lymphotropic method, drug concentration in tissues was higher than after muscle injection, and most antibiotics accumulated in lung tissue within the first hour. Antibiotic kinetics were either linear or logarithmic. Results. The results indicated that the highest concentration of gentamicin sulfate was found in blood samples after intramuscular administration for the first hour, while the least amount of antibiotics accumulated in the pleura. The kinetics of gentamicin were observed to be either linear or logarithmic. The half-life of the drug for lymphotropic administration was found to be 4 hours, while for intramuscular administration, it ranged from 2 to 5 hours, depending on the object of screening. After 24 hours, regardless of the method of administration, the antibiotic was eliminated at residual amounts of no more than 10 μg/mg. Conclusion. The study concluded that the pretracheal method of administration is more effective and can be used to optimize gentamicin treatment of bacterial infections.