Article

The complete chloroplast genome of Lepidium olgae, a narrow endemic species of the Nuratau Range, Uzbekistan

Ibrokhimjon ErgashovTashkent Institute of Botany, Academy of Sciences of UzbekistanFarkhodjon MingboevTashkent Institute of Botany, Academy of Sciences of UzbekistanFarruhbek RasulovAndijan Department of Pharmaceutical Sciences, Andijan State Medical InstituteA. TogaevSurkhandarya Termiz University of Economics and Service, 38-B, Ibn Sino str., Termiz regionKibriyo RasulovaSamarkand Department of Otorhinolaryngology No. 2, Samarkand State Medical UniversityAbdumomin SindorovJizzakh Jizzakh state pedagogical university, 130100, 4 Sharof Rashidov streetMuhayo TagayevaBukhara Muhammad Ikbal Street, 11, 200117, Bukhara State UniversityG‘iyos BuxorovKashkadarya Turon University, 1/14 Nasaf Street, Karshi CityZiyoviddin YusupovTashkent Institute of Botany, Academy of Sciences of UzbekistanSherzod YakhshiboyevSamarkand Samarkand State Architecture and Construction University

F1000Researchjournal2026en

ABI

Abstract

<ns3:p> <ns3:italic>Lepidium olgae</ns3:italic> Regel is a poorly studied Central Asian species of Brassicaceae occurring in arid mountain habitats of the Nuratau Range, Uzbekistan, and its genomic resources have remained limited. In this study, we sequenced, assembled, and characterized the complete chloroplast genome of <ns3:italic>L. olgae</ns3:italic> and evaluated its phylogenetic position within <ns3:italic>Lepidium</ns3:italic> using comparative plastome data from 17 species. The plastome of <ns3:italic>L. olgae</ns3:italic> exhibited the typical circular quadripartite structure of angiosperms, with a total length of 154,837 base pairs. Comparative analysis showed that chloroplast genome sizes among the sampled <ns3:italic>Lepidium</ns3:italic> species ranged from 153,132 to 154,982 base pairs, indicating a high level of structural conservation across the genus. All analyzed plastomes contained 127 unique genes, including 82 protein-coding genes, 37 transfer ribonucleic acid genes, and 8 ribosomal ribonucleic acid genes. Gene content, gene order, and overall genome organization were highly conserved, with only minor variation detected at the boundaries of the large single-copy, small single-copy, and inverted repeat regions. Sliding-window analysis of nucleotide diversity revealed uneven sequence variation across the plastomes, with several highly variable regions, including <ns3:italic>trnQ–psbK</ns3:italic> , <ns3:italic>trnD</ns3:italic> , <ns3:italic>trnL–trnF</ns3:italic> , <ns3:italic>psbJ–psbL</ns3:italic> , <ns3:italic>rpl14–rpl16</ns3:italic> , <ns3:italic>rpl32–ccsA</ns3:italic> , and especially the <ns3:italic>ycf1–trnN</ns3:italic> interval. Phylogenetic analysis based on complete chloroplast genome sequences strongly supported the monophyly of <ns3:italic>Lepidium</ns3:italic> and recovered <ns3:italic>L. olgae</ns3:italic> as a distinct lineage within the genus. These results provide a useful genomic resource for <ns3:italic>Lepidium</ns3:italic> and establish a foundation for future phylogenetic, taxonomic, molecular identification, and conservation-related studies of Central Asian representatives of the genus. </ns3:p>

Topics

Plant Ecology and Taxonomy Studies Genomics and Phylogenetic Studies Photosynthetic Processes and Mechanisms

Identifiers

DOI: 10.12688/f1000research.179708.1

Citations and references

Cited by 039 references

Metrics — AkademScholar · Coming soon