Isolation of 5?- and 5?-dihydrorubrosterone fromSilene otites L. (Wib)
M. B�thoriJean‐Pierre GiraultEcole Normale Supérieure, Département de Biologie, Laboratoire de Biochimie et Physiologie du Developpement, CNRS ER 100, 46 rue d'Ulm, F-75230 Paris, Cedex 05, FranceI. M�th�René LafontUniversité René Descartes, Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, CNRS URA 400, 45 rue des Saint-Péres, F-75006 Paris, France
2000en
ABI
Abstract
5 alpha-Dihydrorubrosterone (2 beta, 3 beta, 14 alpha, 17 beta-tetrahydroxy-5 alpha-androst-7-ene-6-one), a new 19-carbon 5 alpha-ecdysteroid, was isolated together with its 5 beta counterpart from the aerial parts of Silene otites L. (Wib.) (Caryophyllaceae) by a combination of solvent partition, low-pressure column chromatography, thin-layer chromatography (normal-phase and reversed-phase) and finally HPLC. Mass spectrometry and nuclear magnetic resonance spectroscopic procedures were used for compound characterization.
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