HPLC Analysis of Ecdysteroids in Plant Extracts Using Superheated Deuterium Oxide with Multiple On-Line Spectroscopic Analysis (UV, IR, <sup>1</sup>H NMR, and MS)
Dave LoudenLGC, Runcom, Cheshire, UKAlan HandleyRené LafontÉcole Normale Superieure, Départment de Biologie, CNRS EP 119,46 rue d'Ulm, F-75230 Paris, Cedex 05, FranceS. TaylorIan SinclairEva M. LenzTimothy OrtonIan D. Wilson
2001en
ABI
Abstract
HPLC, using superheated D20 as the mobile phase, combined with on-line characterization via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and mass spectrometry has been used for the analysis of a standard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometers enabled the on-flow collection of UV, 1H NMR, IR, and mass spectra not only for pure 20-hydroxyecdysone (100-400 microg on column) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.
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