In Vivo Protein Transduction: Delivery of a Biologically Active Protein into the Mouse
Steven R. SchwarzeHoward Hughes Medical Institute and Departments of Pathology and Medicine, Washington University School of Medicine, St. Louis, MO 63110, USAAlan L. HoHoward Hughes Medical Institute and Departments of Pathology and Medicine, Washington University School of Medicine, St. Louis, MO 63110, USAAdamina Vocero-AkbaniHoward Hughes Medical Institute and Departments of Pathology and Medicine, Washington University School of Medicine, St. Louis, MO 63110, USASteven F. DowdyHoward Hughes Medical Institute and Departments of Pathology and Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA
1999en
ABI
Abstract
Delivery of therapeutic proteins into tissues and across the blood-brain barrier is severely limited by the size and biochemical properties of the proteins. Here it is shown that intraperitoneal injection of the 120-kilodalton beta-galactosidase protein, fused to the protein transduction domain from the human immunodeficiency virus TAT protein, results in delivery of the biologically active fusion protein to all tissues in mice, including the brain. These results open new possibilities for direct delivery of proteins into patients in the context of protein therapy, as well as for epigenetic experimentation with model organisms.
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