Trypan Blue Exclusion Test of Cell Viability
Warren StroberNational Institute of Allergy and Infectious Diseases, NIH Bethesda Maryland
2015en
ABI
Abstract
The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).
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Cited by 20 references