Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Clotilde ThéryInstitut Curie INSERM U932 PSL Research University Paris FranceKenneth W. WitwerThe Johns Hopkins University School of Medicine Department of Molecular and Comparative Pathobiology Baltimore MD USAElena AïkawaBrigham and Women's Hospital Center for Interdisciplinary Cardiovascular Sciences Boston MA USAMaría José AlcarazInteruniversity Research Institute for Molecular Recognition and Technological Development (IDM) University of Valencia Polytechnic University of Valencia Valencia SpainJohnathon D. AndersonUniversity of California, Davis Department of Otolaryngology Davis CA USARamaroson AndriantsitohainaINSERM U1063 Université d'Angers CHU d'Angers Angers FranceAnna AntoniouGerman Centre for Neurodegenerative Diseases (DZNE) Bonn GermanyTanina ArabUniversité de Lille INSERM, U‐1192 Laboratoire Protéomique Réponse Inflammatoire et Spectrométrie de Masse ‐ PRISM Lille FranceFabienne ArcherUniversity of Lyon INRA EPHE UMR754 Viral Infections and Comparative Pathology Lyon FranceGeorgia K. Atkin‐SmithLa Trobe University La Trobe Institute for Molecular Science Department of Biochemistry and Genetics Bundoora AustraliaD. Craig AyreAtlantic Cancer Research Institute Moncton CanadaJean‐Marie BachUniversité Bretagne Loire Oniris INRA IECM Nantes FranceDaniel BachurskiUniversity of Cologne Department of Internal Medicine I Cologne GermanyHossein BaharvandRoyan Institute for Stem Cell Biology and Technology ACECR Cell Science Research Center Department of Stem Cells and Developmental Biology Tehran IranLeonora BalajMassachusetts General Hospital Department of Neurosurgery Boston MA USAShawn BaldacchinoUniversity of Malta Department of Pathology Msida MaltaNatalie BauerUniversity of South Alabama Department of Pharmacology Center for Lung Biology Mobile AL USAAmy A. BaxterLa Trobe University La Trobe Institute for Molecular Science Department of Biochemistry and Genetics Bundoora AustraliaMary BebawyUniversity of Technology Sydney Discipline of Pharmacy Graduate School of Health Sydney AustraliaCarla BeckhamUniversity of Rochester Rochester NY USAApolonija Bedina ZavecNational Institute of Chemistry Department of Molecular Biology and Nanobiotechnology Ljubljana SloveniaAbderrahim BenmoussaUniversité Laval Centre de Recherche du CHU de Québec Department of Infectious Diseases and Immunity Quebec City CanadaAnna C. BerardiOspedale Santo Spirito Pescara ItalyPaolo BergeseCSGI ‐ Research Center for Colloids and Nanoscience Florence ItalyEwa BielskaUniversity of Birmingham Institute of Microbiology and Infection Birmingham UKCherie BlenkironUniversity of Auckland Auckland New ZealandSylwia Bobis‐WozowiczJagiellonian University Faculty of Biochemistry, Biophysics and Biotechnology Department of Cell Biology Kraków PolandÉric BoilardUniversité Laval Centre de Recherche du CHU de Québec Department of Infectious Diseases and Immunity Quebec City CanadaWilfrid BoireauFEMTO‐ST Institute UBFC CNRS ENSMM UTBM Besançon FranceAntonella BongiovanniInstitute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) of Italy Palermo ItalyFrancesc E. BorràsGermans Trias i Pujol Research Institute (IGTP) Can Ruti Campus REMAR‐IVECAT Group Badalona SpainSteffi BöschUniversité Bretagne Loire Oniris INRA IECM Nantes FranceChantal M. BoulangerINSERM UMR‐S 970 Paris Cardiovascular Research Center Paris FranceXandra O. BreakefieldMassachusetts General Hospital and Neuroscience Program Harvard Medical School Department of Neurology and Radiology Boston MA USAAndrew BreglioIcahn School of Medicine at Mount Sinai New York City NY USAMeadhbh Á. BrennanHarvard University School of Engineering and Applied Sciences Cambridge MA USADavid R. BrigstockNationwide Children's Hospital Columbus OH USAAlain BrissonUMR‐CBMN CNRS‐Université de Bordeaux Bordeaux FranceMarike L. D. BroekmanHaaglanden Medical Center Department of Neurosurgery The Hague The NetherlandsJacqueline BrombergMemorial Sloan Kettering Cancer Center Department of Medicine New York City NY USAPaulina Bryl‐GóreckaLund University Department of Cardiology Lund SwedenShilpa BuchUniversity of Nebraska Medical Center Department of Pharmacology and Experimental Neuroscience Omaha NE USAAmy H. BuckUniversity of Edinburgh Institute of Immunology & Infection Research Edinburgh UKDylan BurgerKidney Research Centre Ottawa CanadaSara BusattoMayo Clinic Department of Transplantation Jacksonville FL USADominik BuschmannTechnical University of Munich TUM School of Life Sciences Weihenstephan Division of Animal Physiology and Immunology Freising GermanyBenedetta BussolatiUniversity of Torino Department of Molecular Biotechnology and Health Sciences Torino ItalyEdit I. BuzásMTA‐SE Immuno‐Proteogenomics Research Groups Budapest HungaryJames Brian ByrdUniversity of Michigan Department of Medicine Ann Arbor MI USAGiovanni CamussiUniversity of Torino Department of Medical Sciences Torino ItalyDavid R. F. CarterOxford Brookes University Department of Biological and Medical Sciences Oxford UKSarah CarusoLa Trobe University La Trobe Institute for Molecular Science Department of Biochemistry and Genetics Bundoora AustraliaLawrence W. ChamleyUniversity of Auckland Department of Obstetrics and Gynaecology Auckland New ZealandYu‐Ting ChangNational Taiwan University Hospital Department of Internal Medicine Taipei TaiwanChihchen ChenNational Tsing Hua University Department of Power Mechanical Engineering Hsinchu TaiwanDaiwen ChenLeibniz Institute for Farm Animal Biology (FBN) Institute of Reproductive Biology Dummerstorf GermanyLesley ChengLa Trobe University La Trobe Institute for Molecular Science Department of Biochemistry and Genetics Bundoora AustraliaAndrew R. ChinCedars‐Sinai Medical Center Los Angeles CA USAAled ClaytonCardiff University School of Medicine Cardiff UKStefano Piatto ClericiUNICAMP Institute of Biology Campinas BrazilAlex CocksCardiff University School of Medicine Cardiff UKEmanuele CocucciThe Ohio State University College of Pharmacy Division of Pharmaceutics and Pharmaceutical Chemistry Columbus OH USARobert J. CoffeyVanderbilt University Medical Center Epithelial Biology Center Department of Medicine Nashville TN USAAnabela Cordeiro‐da‐SilvaUniversity of Porto Faculty of Pharmacy (FFUP) IBMC/I3S Porto PortugalYvonne CouchUniversity of Oxford Radcliffe Department of Medicine, Acute Stroke Programme ‐ Investigative Medicine Oxford UKFrank A. W. CoumansAcademic Medical Centre of the University of Amsterdam Department of Clinical Chemistry and Vesicle Observation Centre Amsterdam The NetherlandsBeth CoyleThe University of Nottingham School of Medicine Children's Brain Tumour Research Centre Nottingham UKRossella CrescitelliUniversity of Gothenburg Institute of Medicine at Sahlgrenska Academy Krefting Research Centre Gothenburg SwedenMiriã Ferreira CriadoUniversity of São Paulo Ribeirão Preto Medical School Ribeirão Preto BrazilCrislyn D’Souza‐SchoreyUniversity of Notre Dame Department of Biological Sciences Notre Dame IN USASaumya DasMassachusetts General Hospital Boston MA USAAmrita Datta ChaudhuriThe Johns Hopkins University School of Medicine Department of Neurology Baltimore MD USAPaola de CandiaIRCCS MultiMedica Milan ItalyEliezer F De SantanaThe Sociedade Beneficente Israelita Brasileira Albert Einstein São Paulo BrazilOlivier De WeverCancer Research Institute Ghent Ghent BelgiumHernando A. del PortilloISGlobal Hospital Clínic ‐ Universitat de Barcelona PVREX Group Barcelona SpainTanguy DemaretUniversité Catholique de Louvain Institut de Recherche Expérimentale et Clinique (IREC) Laboratory of Pediatric Hepatology and Cell Therapy Brussels BelgiumSarah DevilleUniversiteit Hasselt Diepenbeek BelgiumAndrew DevittAston University School of Life & Health Sciences Birmingham UKBert DhondtCancer Research Institute Ghent Ghent BelgiumDolores Di VizioCedars‐Sinai Medical Center Los Angeles CA USALothar C. DieterichETH Zurich Institute of Pharmaceutical Sciences Zurich SwitzerlandVincenza DoloUniversity of L'Aquila Department of Life, Health and Environmental Sciences L'Aquila ItalyAna Paula Domínguez RubioUniversidad de Buenos Aires Facultad de Ciencias Exactas y Naturales Departamento de Química Biológica Buenos Aires ArgentinaMassimo DominiciTPM of Mirandola Mirandola ItalyMaurício Rocha DouradoUniversity of Campinas Piracicaba Dental School Department of Oral Diagnosis Piracicaba BrazilTom A. P. DriedonksUtrecht University Faculty of Veterinary Medicine Department of Biochemistry and Cell Biology Utrecht The NetherlandsFilipe V. DuarteHeather M. DuncanMcGill University Division of Experimental Medicine Montreal CanadaRamon M. EichenbergerJames Cook University Australian Institute of Tropical Health and Medicine Centre for Biodiscovery and Molecular Development of Therapeutics Cairns AustraliaKarin M. EkströmUniversity of Gothenburg Institute of Clinical Sciences at Sahlgrenska Academy Department of Biomaterials Gothenburg SwedenSamir EL AndaloussiEvox Therapeutics Limited Oxford UKCéline Élie-CailleFEMTO‐ST Institute UBFC CNRS ENSMM UTBM Besançon FranceUta ErdbrüggerUniversity of Virginia Health System Department of Medicine Division of Nephrology Charlottesville VA USAJuan Manuel Falcón‐PérezCIC bioGUNE CIBERehd Exosomes Laboratory & Metabolomics Platform Derio SpainFarah FatimaUniversity of São Paulo Ribeirão Preto Medical School Department of Pathology and Forensic Medicine Ribeirão Preto BrazilJason E. FishToronto General Hospital Research Institute University Health Network Toronto CanadaMiguel Flores‐BellverUniversity of Colorado School of Medicine Department of Ophthalmology, Cell Sight‐Ocular Stem Cell and Regeneration Program Aurora CO USAAndrás FörsönitsSemmelweis University Department of Genetics, Cell‐ and Immunobiology Budapest HungaryAnnie Frelet‐BarrandFEMTO‐ST Institute UBFC CNRS ENSMM UTBM Besançon France
2018en
ABI
Abstract
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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Cited by 60 references