Article

Bacterial endophyte Sphingomonas sp. LK11 produces gibberellins and IAA and promotes tomato plant growth

Abdul Latif KhanDepartment of Biological Sciences and Chemistry, University of Nizwa, Nizwa, OmanMuhammad WaqasSchool of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of KoreaSang‐Mo KangSchool of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of KoreaAhmed Al‐HarrasiDepartment of Biological Sciences and Chemistry, University of Nizwa, Nizwa, OmanJavid HussainDepartment of Biological Sciences and Chemistry, University of Nizwa, Nizwa, OmanAhmed Al‐RawahiDepartment of Biological Sciences and Chemistry, University of Nizwa, Nizwa, OmanSalima Al-KhiziriDepartment of Biological Sciences and Chemistry, University of Nizwa, Nizwa, OmanIhsan UllahSchool of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of KoreaLiaqat AliDepartment of Biological Sciences and Chemistry, University of Nizwa, Nizwa, OmanHee–Young JungSchool of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of KoreaIn‐Jung LeeSchool of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

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ABI

Abstract

Plant growth promoting endophytic bacteria have been identified as potential growth regulators of crops. Endophytic bacterium, Sphingomonas sp. LK11, was isolated from the leaves of Tephrosia apollinea. The pure culture of Sphingomonas sp. LK11 was subjected to advance chromatographic and spectroscopic techniques to extract and isolate gibberellins (GAs). Deuterated standards of [17, 17-2H2]-GA4, [17, 17-2H2]-GA9 and [17, 17-2H2]-GA20 were used to quantify the bacterial GAs. The analysis of the culture broth of Sphingomonas sp. LK11 revealed the existence of physiologically active gibberellins (GA4: 2.97 ± 0.11 ng/ml) and inactive GA9 (0.98 ± 0.15 ng/ml) and GA20 (2.41 ± 0.23). The endophyte also produced indole acetic acid (11.23 ± 0.93 μM/ml). Tomato plants inoculated with endophytic Sphingomonas sp. LK11 showed significantly increased growth attributes (shoot length, chlorophyll contents, shoot, and root dry weights) compared to the control. This indicated that such phyto-hormones-producing strains could help in increasing crop growth.

Identifiers

DOI: 10.1007/s12275-014-4002-7

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