HPLC analysis and blood-brain penetration of 20-hydroxyecdysone diacetonide
Huba KalászDepartment of Pharmacology and Pharmacotherapy, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, HungaryAttila HunyadiInstitute of Pharmacognosy, University of Szeged, 6720 Szeged, Eötvös u. 6, HungaryKornélia TekesDepartment of Pharmacodynamics, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, HungaryRafael DolesalUniversity Hospital Hradec Kralove, 500 05 Hradec Kralove, Sokolska 581, Czech RepublicGellért KarvalyDepartment of Laboratory Medicine, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary
2016en
ABI
Abstract
Blood-brain penetration of 20-hydroxyecdysone 2,3;20,22-diacetonide (20DA) has been scouted using chromatographic methods. In vivo experiments were performed by treating male Wistar rats intraperitoneally (i.p.) with a dose of 50 mg kg −1 20DA. Control experiment was done by using 20-hydroxyecdysone (20E). Definite brain penetration of 20DA was found by using high-performance liquid chromatography (HPLC), while 20E does not show this type of distribution.
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