Article

Ultrasensitive optoelectronic biosensor arrays based on twisted bilayer graphene superlattice

Bowen DuCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060Xilin TianCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060Zhi ChenCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060Yanqi GeCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060Chuanghu ChenCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060Haiyan GaoIntensive Care Unit, Shenzhen Key Laboratory of Microbiology in Genomic Modification & Editing and Application, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Department of Biomedical Engineering, , Shenzhen 518000Zhongyang LiuThe Photonics Center, Shenzhen University , Shenzhen 518060Jung‐Chen TungTaipei University of Technology Department of Electro-Optical Engineering, , Taipei 10608Dror FixlerBar-Ilan University Department of Engineering, , Ramat Gan 52001Songrui WeiCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060Shi ChenIntensive Care Unit, Shenzhen Key Laboratory of Microbiology in Genomic Modification & Editing and Application, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Department of Biomedical Engineering, , Shenzhen 518000Han ZhangCollege of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060

2025en

ABI

Abstract

Recent advances in twistronics have revealed tunable optoelectronic properties in twisted bilayer graphene (tBLG), including angle-dependent dielectric responses and enhanced light absorption due to van Hove singularity (VHS). However, achieving high photoresponsivity in tBLG-based sensors typically requires intense illumination. We present an ultrasensitive optoelectronic biosensor integrating tBLG superlattices with Au nanodisks and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a via DNA origami. By aligning the 9.4° tBLG's VHS absorption spectrum with Au nanodisks' plasmonic resonance at 60 μW, we achieve a 7-fold photocurrent enhancement over pristine tBLG. CRISPR-Cas12a-mediated trans-cleavage dynamically modulates the local dielectric environment, enabling sub-femtomolar (44.63 attomolar, aM) nucleic acid detection without external amplification. Clinical validation using lung cancer samples shows high concordance with quantitative polymerase chain reaction (qPCR), demonstrating real-time, label-free detection of microRNA (miRNA). This hybrid platform combines moiré-engineered optoelectronics with programmable bio-nanoarrays, offering a scalable solution for precision diagnostics with ultralow detection limits and rapid response times.

Identifiers

DOI: 10.1093/nsr/nwaf357

Citations and references

Cited by 80 references

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