Article

De novo assembly of the <i>Aedes aegypti</i> genome using Hi-C yields chromosome-length scaffolds

Olga DudchenkoCenter for Theoretical and Biological Physics, Rice University, Houston, TX 77030, USASanjit Singh BatraDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USAArina D. OmerDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USASarah K. NyquistDepartments of Computer Science and Computational and Applied Mathematics, Rice University, Houston, TX 77030, USAMarie HoegerDepartments of Computer Science and Computational and Applied Mathematics, Rice University, Houston, TX 77030, USANeva C. DurandDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USAMuhammad S. ShamimDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USAIdo MacholDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USAEric S. LanderBroad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USAAviva Presser AidenDepartment of Bioengineering, Rice University, Houston, TX 77030, USAErez Lieberman AidenBroad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA

2017en

ABI

Abstract

Hi-C for mosquito genomes Most genomes sequenced today are determined through the generation of short sequenced bits of DNA that are computationally pieced together like a jigsaw puzzle. This has resulted in the need for funds and additional data to fill in gaps in order to fully assemble the many chromosomes that make up a eukaryotic genome. Dudchenko et al. used the Hi-C method, which measures the distance between contact points within and between chromosomes for scaffold validation, together with correction and ordering to more completely determine the arrangement of short sequencing reads for genome mapping. They validated their approach through the de novo generation of a complete human genome. A comparative analysis of mosquito genomes was made possible by improving the Culex quinquefasciatus genome assembly and generating the genome of Aedes aegypti , the vector of Zika virus. Science , this issue p. 92

Identifiers

DOI: 10.1126/science.aal3327

Citations and references

Cited by 40 references

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