Extraction of Glycyrrhizic Acid and Glabridin from Licorice
Minglei TianCenter for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, KoreaHongyuan YanCenter for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, KoreaKyung Ho RowCenter for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea
2008en
ABI
Abstract
The extraction and separation conditions of glycyrrhizic acid and glabridin from licorice were investigated. By changing the different extraction solvents, procedures, times and temperature, the optimum extraction condition was established: the used of ethanol/water (30:70, v/v) as an extraction solvent, and 60 min dipping time under 50 degrees C. The extracts of licorice were separated and determined by reversed-phase high performance liquid chromatography with a methanol/water (70:30, v/v, containing 1% acetic acid) as the mobile phase. Under the optimum extraction condition, 2.39 mg/g of glycyrrhizic acid and 0.92 mg/g of glabridin were extracted from Chinese licorice and the recoveries were 89.7% and 72.5% respectively.
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