DNA sequencing with chain-terminating inhibitors
Frederick SangerMedical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, EnglandS. NicklenMedical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, EnglandAlan CoulsonMedical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England
1977en
ABI
Abstract
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.
Identifiers
Citations and references
Cited by 40 references