Invertase in Immobilized Cells of Eschscholtzia Californica
K. NeubertInstitute of Biochemistry, M. Luther University, Halle, GermanyJ. StanoFaculty of Pharmacy, Comenius University Bratislava, Slovak RepublicKarol MičietaFaculty of Natural Sciences, Comenius University, Bratislava, Slovak RepublicPéter KovácsFaculty of Pharmacy, Comenius University Bratislava, Slovak RepublicH. TintemannInstitute of Biochemistry, M. Luther University, Halle, Germany
2002en
ABI
Abstract
Cell suspension culture of Eschscholtzia californica Cham. were permeabilized by Tween 20 or 80, and immobilized by glutaraldehyde. The highest invertase activity was at pH 4.5 and temperature 50 °C. The hydrolysis of the substrate was linear for 5 h reaching 60 % conversion. The cells had high invertase activity and a good stability, and in long-term storage they showed convenient physico-mechanical properties.
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Cited by 20 references