Plant cell culture : a practical approach
Abstract
1: Isolation and maintenance of callus and cell suspension cultures. 2: Isolation, culture, and regeneration of protoplasts. 3: Applications of protoplast technology. 3.A: Fusion and selection of somatic hybrids. 3.B: Transient gene expression and stable transformation. 3.C: Studies with viruses. 4: Selection of plant cells for desirable characteristics. 4.A: Inhibitor resistance. 4.B: Cold tolerance. 4.C: In vitro selection for salt tolerance. 4.D: In vitro selection for disease/toxin resistance. 5: Plant regeneration via embryonic suspension cultures. 6: Applied aspects of plant regeneration. 6.A: Micropropagation. 6.B: Virus-free plants. 6.C: Artificial seeds. 7: Cryopreservation. 8: Secondary products from cultured cells and organs I: molecular and cellular approaches. 9: Secondary products from cultured cells and organs II: large scale culture