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Assay of intestinal disaccharidases

Arne DahlqvistDepartment of Nutrition, Chemistry Centre, University of Lund, Box 740, S-220 07, Lund, Sweden
1984en
ABI

Abstract

A modified and updated description of the author's method for the assay of intestinal disaccharidases. The principle of the method is the following: an intestinal homogenate is incubated with the appropriate disaccharide. The disaccharidase activity is then interrupted by the addition of TRIS, and the glucose liberated is measured with a glucose oxidase reagent.

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