Article

Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa

Zoltán BéniSpectroscopic Research, Gedeon Richter Plc., Gyömrői út 19-21, H-1103 Budapest, HungaryMiklós DékánySpectroscopic Research, Gedeon Richter Plc., Gyömrői út 19-21, H-1103 Budapest, HungaryKovács BernadettDepartment of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryBoglárka Csupor‐LöfflerDepartment of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryZoltán Péter ZomborszkiDepartment of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryErika Beáta KerekesDepartment of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, HungaryAndrás SzekeresDepartment of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, HungaryEdit UrbánInstitute of Clinical Microbiology, University of Szeged, Semmelweis u. 6, H- 6725 Szeged HungaryJudit HohmannDepartment of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryAttila VányolósDepartment of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary

2018en

ABI

Abstract

Bioassay-guided fractionation of the chloroform extract of Tapinella atrotomentosa led to the isolation of four secondary metabolites 1–4. Two of the compounds are lactones—osmundalactone (1) and 5-hydroxy-hex-2-en-4-olide (2)—while 3 and 4 were identified as terphenyl quinones, spiromentins C and B, respectively. The structures of the compounds were established on the basis of NMR and MS spectroscopic analysis. The isolated fungal metabolites were evaluated for their antibacterial activities against several Gram-positive and negative bacteria. In addition, their synergistic effect with cefuroxime against methicillin-resistant Staphylococcus aureus (MRSA) was also evaluated. Compounds 1–3 proved to possess significant antibacterial activity against multiresistant Acinetobacter baumannii and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The investigation of the antioxidant effect of the isolated compounds in DPPH and ORAC assays revealed that spiromentins C (3) and B (4) have remarkable antioxidant activity.

Identifiers

DOI: 10.3390/molecules23051082

Citations and references

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