RNA isolation from siliques, dry seeds and other tissues of Arabidopsis thaliana

Arabidopsis RNA Isolation Plants often present a challenge to researchers wanting to isolate nucleic acids for subsequent analyses as a result of the high amounts of polysaccharides and other contaminating metabolites present in plant tissues. Suzuki et al. (p. 542) describe an isolation method that produces RNA free of contamination by protein or polysaccharides or degradation, suitable for reverse transcription PCR (RT-PCR). The authors extracted RNA from Arabidopsis siliques, seeds, flower buds, leaves, roots, and stems with no decrease in the quality or quantity of RNA. Their method was both time- and cost-effective over more conventional methods and is simple enough to be routinely applied to other species.

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