Статья

Molecular Epidemiology and Interferon Susceptibility of the Natural Recombinant Hepatitis C Virus Strain RF1_2k/1b

Fuat KurbanovDepartment of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, JapanYasuhito TanakaDepartment of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, andE. V. ChubDepartment of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, andIsao MaruyamaPhoenixBio, Hiroshima, andАзиза АзлароваDepartment of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, andHiroshi KamitsukasaDepartment of Gastroenterology, Tokyo National Hospital, Tokyo, Japan;Tomoyoshi OhnoDepartment of Gastroenterology, Social Insurance, Chukyo Hospital, Nagoya,Stefania BonettoInstitute of Hepatology, 69 -75 Chenies Mews, University College London, London, United Kingdom;Isabelle MoreauMolecular Virology Diagnostic and Research Laboratory, Department of Medicine, Cork University Hospital/University College Cork, Cork, Republic of Ireland; andLiam J. FanningMolecular Virology Diagnostic and Research Laboratory, Department of Medicine, Cork University Hospital/University College Cork, Cork, Republic of Ireland; andFlorence AbravanelINSERM U563-IFR30 and Laboratoire de Virologie, Institut Fdratif de Biologie de Purpan, Toulouse, FranceJaques IzopetINSERM U563-IFR30 and Laboratoire de Virologie, Institut Fdratif de Biologie de Purpan, Toulouse, FranceNikolai V. NaoumovInstitute of Hepatology, 69 -75 Chenies Mews, University College London, London, United Kingdom;Takashi ShimadaPhoenixBio, Hiroshima, andNetesov SvState Research Center of Virology and Biotechnology VECTOR Koltsovo, Novosibirsk Region, Russian Federation;Masashi MizokamiDepartment of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, and

The Journal of Infectious Diseasesjournal2008en

ABI

Аннотация

BACKGROUND: Hepatitis C virus (HCV) genotype is an important determinant of virological response to antiviral therapies. Currently, there are no data available on the molecular epidemiology and interferon susceptibility of the natural intergenotypic recombinant RF1_2k/1b (RF1) strain. METHODS: Genotyping and RF1-PCR screening were performed on samples from 604 HCV RNA-positive individuals from 7 countries. uPA/SCID mice carrying human hepatocytes (chimeric mice) were infected with the RF1_2k/1b strain, and the susceptibility of the strain to interferon and ribavirin was compared with the susceptibilities of 2 different strains of genotype B, used as references. RESULTS: Six new RF1 cases were identified in this study; 5 (2%) of 281 in Russia and 1 (1%) of 90 in Uzbekistan. Phylogenetic analyses based on Core/E1 and NS5b indicated that all RF1 representatives share a common evolutionary ancestor. Infection with RF1 was established in chimeric mice. Reduction of RF1 viral load was observed in response to 3 injections of 3 microg/kg pegylated-interferon alpha-2a alone or in combination with 50 mg/kg of ribavirin (0.5 or 1.4 log-copies/mL). CONCLUSIONS: All identified RF1-type strains appear to be introduced from a single source, suggesting that intergenotypic recombination in HCV is sporadic and not associated with cocirculation of different genotypes in a population. The RF1 strain in this study was responsive to interferon in vivo.

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Темы

Hepatitis C virus research HIV/AIDS drug development and treatment Hepatitis Viruses Studies and Epidemiology

Идентификаторы

DOI: 10.1086/592757

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