Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity
Maja MiloševićLaboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, SerbiaSnježana PetrovićLaboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, SerbiaIvana StanojevićLaboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, SerbiaDunja DrakulićLaboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, SerbiaNataša VeličkovićLaboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, SerbiaAnica HorvatLaboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, Serbia
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Аннотация
The aim of this study was to examine in vitro chelator’s ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO4 concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC50 values, 1.15 × 10−4 and 1.71 × 10−3 mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO4 concentration for half-maximal inhibition (IC50), by decreasing Cu2+ concentrations, available to form inactive CuATP2− complex.
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