The spectroscopic manifestation of the interaction of some cyanine dyes with BSA and DNA
Eldar N. KurtalievSamarkand State Univ. (Uzbekistan)Negmat NizomovSamarkand State Univ. (Uzbekistan)Sherzod I. RahimovSamarkand State Univ. (Uzbekistan)Gayrat KhodjayevSamarkand Agricultural Institute (Uzbekistan)Dilorom P. Khakimova
Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIEjournal2010en
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Аннотация
The spectral-luminescent characteristics of cyanine dye Cyan 40 and thiazole orange at interaction with biomacromolecules bovine serum albumin and deoxyribonucleic acid was studied. It is shown that presence of biomacromolecules in solution of the studied species leads to the change of spectral-luminescent characteristics of the dyes Cyan 40 and TO. The observed phenomena in absorption spectrum and fluorescence of the studied dyes is explained by complex formation between dye and biological macromolecules. The binding parameters: a binding constant (K) and quantity of the binding sites (N) the studied dyes with biomacromolecules are determined.
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