Статья

NANODEVICE-BASED GENOME EDITING TO ENHANCE CRISPR/CAS9 STABILITY AND EFFICIENCY

Gopika K MoteState Agriculture University , Akola -444104 , Maharashtra State , IndiaP.V. JadhavState Agriculture University , Akola -444104 , Maharashtra State , IndiaShivani S. PawarState Agriculture University , Akola -444104 , Maharashtra State , IndiaS.P. NagraleState Agriculture University , Akola -444104 , Maharashtra State , IndiaArpita MahobiaState Agriculture University , Akola -444104 , Maharashtra State , IndiaUmesh ShindeState Agriculture University , Akola -444104 , Maharashtra State , IndiaM.P. MoharilState Agriculture University , Akola -444104 , Maharashtra State , IndiaS.D. JadhaoState Agriculture University , Akola -444104 , Maharashtra State , IndiaSanjay B. SakhareState Agriculture University , Akola -444104 , Maharashtra State , IndiaGhorade GhoradeS.S. ManeState Agriculture University , Akola -444104 , Maharashtra State , IndiaDeshmukh PanjabraoRavipraksh G DaniTermez State University , Termez , Republic of Uzbekistan

Вестник Ошского государственного университета Химия Биология Географияjournal2022en

ABI

Аннотация

Since a decade ago, the importance of CRISPR and the CRISPR-associated system (Cas) in the field of genome modification has increased. The limited intracellular delivery effectiveness of this method makes it difficult to transport Cas payloads and sgRNA despite its adaptability. Nanomaterials including liposomes, polymers, gold nanoparticles, and inorganic nanoparticles have been used successfully for gene transfer. Here, we briefly cover the many CRISPR/Cas delivery systems and their related difficulties, then we go through the different nanotechnological ways for CRISPR/Cas delivery, and look at the numerous issues that CRISPR-based plant genome editing encounters. It also covered the challenges of delivering CRISPR/Cas9 utilising nanotechnology and the regions that must be targeted to benefit from this editing approach.

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Темы

CRISPR and Genetic Engineering Advanced biosensing and bioanalysis techniques RNA Interference and Gene Delivery

Идентификаторы

DOI: 10.52754/16948688_2022_1_8

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