An approach to the classification of forest resources taking into account modern economic challenges

(Populus ssp.) - LEAFY (LFY) AGAMOUS (AG) [42]. P. trichocarpa , , , 60-65 . 8 P. trichocarpa () [39]. , AGAMOUS (AG) , LEAFY -.Elorriaga .[14] ( ) (single guide RNA, sgRNA), CRISPR/Cas9 () LFY AG. . , -. , CRISPR/ Cas9 . , C R I S P R / C a s Agrobacterium- , . 550 Mb ( ) [39], : P. trichocarpa [39], P. euphratica [23], P. tremula [22] P. deltoides [5]. Populus [9]. , , PLFY P. trichocarpa (GenBank accession number U93196, Potri.015G106900), LEAFY Arabidopsis, Phytozome [17]. - CRISPR/Cas9 Cas9 (CRISPR associated protein 9) - , . , cr (CRISPR ). Cas9 -tracr (trans-activating CRISPR ), cr. Cas9/ cr/tracr , 20 5'- cr. ( ). , 5'- 20- () PAM-, Cas (, CCN, Cas9 Streptococcus pyogenes). PAM-, Cas9 , 3 4 ( 3'-5' PAM-). . - cr (CRISPR ), , (tracrRNA) -- , Cas9. cr -sgRNA (single guide RNA scaffold). -, , 17-20 .

Перевод пока недоступен