Determination of norfloxacin by sensitized terbium fluorescence in the presence of silver nanoparticles and surfactant micelles

The influence of silver nanoparticles and surfactant micelles on the signal intensity of the sensitized fluorescence of the norfloxacin complex with the Tb3+ ion was studied. It was shown that the intensity of the intrinsic fluorescence of norfloxacin and its complex with Tb3+ ions slightly decreases in the presence of silver nanoparticles. It was found that the presence of dodecyl sulfate micelles leads to the modification of the silver nanoparticle surface, which is accompanied by a change in the ζ-potential of the nanoclusters. The addition of surfactant-modified silver nanoparticles to aqueous solutions of terbium chelate increases the intensity of sensitized fluorescence by 11 times. The increase in the efficiency of intramolecular energy transfer in the Tb3+ complex with norfloxacin in the presence of silver nanoparticles and dodecyl sulfate micelles made it possible to propose a method for the fluorimetric determination of the antibiotic in natural water in the concentration range of 2 × 10-7 −1 × 10-5 M. The calibration graph equation is y = 1.0 × 107x − 1.0, R2 = 0.991, detection limit 5 × 10–8 M (3σ).

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