Препринт

Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

Martina MarcotulliCenter for Life Nano Neuro Science CLN2S, Italian Institute of Technology (IIT);Arianna IacominoCenter for Life Nano Neuro Science CLN2S, Italian Institute of Technology (IIT);Federico SerpeDepartment of Chemistry, Sapienza University of Rome;Lucia IafrateDepartment of Mechanical and Aerospace Engineering (DIMA), Sapienza University of Rome;Marco BastioliCenter for Life Nano Neuro Science CLN2S, Italian Institute of Technology (IIT);Giorgia MontalbanoDepartment of Applied Science and Technology, Polytechnic University of Turin;Biagio PalmisanoDepartment of Molecular Medicine, Sapienza University of Rome;Silvia FrancoInstitute for Complex Systems, National Research Council (ISC-CNR), Sapienza site, Piazzale Aldo Moro, 5, 00185 Rome Italy and Physics Department, Sapienza University of RomeRoberta AngeliniInstitute for Complex Systems, National Research Council (ISC-CNR), Sapienza site, Piazzale Aldo Moro, 5, 00185 Rome Italy and Physics Department, Sapienza University of RomeAlessandro CorsiDepartment of Molecular Medicine, Sapienza University of Rome;Mara RiminucciDepartment of Molecular Medicine, Sapienza University of Rome;Giancarlo RuoccoCenter for Life Nano Neuro Science CLN2S, Italian Institute of Technology (IIT);Chiara ScognamiglioCenter for Life Nano Neuro Science CLN2S, Italian Institute of Technology (IIT);Andrea BarbettaDepartment of Chemistry, Sapienza University of Rome;Gianluca CidonioDepartment of Mechanical and Aerospace Engineering (DIMA), Sapienza University of Rome;

bioRxiv (Cold Spring Harbor Laboratory)repository2025en

ABI

Аннотация

Abstract The unparalleled ability of aqueous two-phase systems (ATPS) to reproduce microscale cellular and biomaterial compartmentalisation to selectively modulate cell behaviour and functionality is ideal for tissue engineering and regenerative medicine (TERM) purposes. Herein, we introduce new ATPS biomaterial inks for 3D bioprinting of water-in-water (W/W) emulsions, enabling precise cellular crowding for tissue regeneration in vitro and ex vivo . Gelatin methacryloyl (GelMA) was hierarchically dispersed in an alginic acid phase depending on sodium chloride (NaCl) concentration (0-36 g/L). Emulsion droplet size (12.8±2.6 µm to 52.4±11.4 µm) influenced degradation and spatial cell localisation (A549, C2C12, MG63). A microfluidic-assisted 3D bioprinting approach allowed fine-tuning of fibre structure adjusting ATPS deposition by modulating flow rates and printing speed. Rheological properties supported the findings of the two-phase partitioning, aiding the selection of the ATPS ink formulation for functional cell-laden construct fabrication. Encapsulation of C2C12 cells revealed enhanced cytoskeletal remodelling at higher salt concentrations. Increased GelMA phase promoted human bone marrow stromal cells (HBMSCs) crowding, mineral deposition and skeletal differentiation. In ovo studies demonstrated degradation control and vascular infiltration via salt modulation. Altogether, ATPS bioinks offer a versatile platform for the assembling of complex, hierarchical tissues with microscale precision, expanding biofabrication strategies for TERM applications.

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Темы

3D Printing in Biomedical Research Innovative Microfluidic and Catalytic Techniques Innovation bioluminescence and chemiluminescence research

Идентификаторы

DOI: 10.1101/2025.06.27.661968

Цитирования и источники

Цитирований: 0Использованных источников: 49

Показатели — AkademScholar