Статья

Aqueous Two‐Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells

Martina MarcotulliCenter for Life Nano‐ & Neuro‐ Science – CLN2S Italian Institute of Technology (IIT) Rome ItalyArianna IacominoCenter for Life Nano‐ & Neuro‐ Science – CLN2S Italian Institute of Technology (IIT) Rome ItalyFederico SerpeDepartment of Chemistry Sapienza University of Rome Rome ItalyLucia IafrateCenter for Life Nano‐ & Neuro‐ Science – CLN2S Italian Institute of Technology (IIT) Rome ItalyMarco BastioliCenter for Life Nano‐ & Neuro‐ Science – CLN2S Italian Institute of Technology (IIT) Rome ItalyGiorgia MontalbanoDepartment of Applied Science and Technology Polytechnic University of Turin Turin ItalyBiagio PalmisanoDepartment of Information Engineering Tor Vergata University of Rome Rome ItalySilvia FrancoDepartment of Molecular Medicine Sapienza University of Rome Rome ItalyRoberta AngeliniInstitute for Complex Systems National Research Council (ISC‐CNR) Rome ItalyAlessandro CorsiDepartment of Information Engineering Tor Vergata University of Rome Rome ItalyMara RiminucciInstitute for Complex Systems National Research Council (ISC‐CNR) Rome ItalyG. RuoccoDepartment of Information Engineering Tor Vergata University of Rome Rome ItalyChiara ScognamiglioDepartment of Molecular Medicine Sapienza University of Rome Rome ItalyAndrea BarbettaDepartment of Molecular Medicine Sapienza University of Rome Rome ItalyGianluca CidonioCenter for Life Nano‐ & Neuro‐ Science – CLN2S Italian Institute of Technology (IIT) Rome Italy

Advanced Healthcare Materialsjournal2026en

ABI

Аннотация

The unparalleled ability of aqueous two-phase systems (ATPS) to reproduce microscale cellular and biomaterial compartmentalization to selectively modulate cell behavior and functionality is ideal for tissue engineering and regenerative medicine (TERM) purposes. Herein, we introduce new ATPS biomaterial inks for 3D bioprinting of water-in-water (W/W) emulsions, enabling precise cellular crowding for tissue regeneration in vitro and ex vivo. Gelatin methacryloyl (GelMA) was dispersed in an alginic acid phase depending on sodium chloride (NaCl) concentration (0-36 g/L). Emulsion droplet size (12.8 ± 2.6-52.4 ± 11.4 µm) influenced degradation and spatial cell localization (A549, C2C12, MG63). A microfluidic-assisted 3D bioprinting approach allowed fine-tuning of fiber structure adjusting ATPS deposition by modulating flow rates and printing speed. Rheological properties supported the findings of the two-phase partitioning, aiding the selection of the ATPS ink formulation for functional cell-laden construct fabrication. Encapsulation of C2C12 cells revealed enhanced cytoskeletal remodeling at higher salt concentrations. Increased GelMA phase promoted human bone marrow stromal cells (HBMSCs) crowding, mineral deposition and skeletal differentiation. In ovo studies demonstrated degradation control and vascular infiltration via salt modulation. Altogether, ATPS bioinks offer a versatile platform for the assembling of complex, hierarchical tissues with microscale precision, expanding biofabrication strategies for TERM applications.

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Темы

3D Printing in Biomedical Research Innovative Microfluidic and Catalytic Techniques Innovation Bacterial biofilms and quorum sensing

Идентификаторы

DOI: 10.1002/adhm.202503219

Цитирования и источники

Цитирований: 0Использованных источников: 52

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