Статья

A novel C5- <i>O</i> -methyltransferase for naringenin refines the biosynthetic strategy for polymethoxyflavones

Honglu HuLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Zhenkun LiaoLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Chenwen ZhouLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Bin LiaoLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Xiaojuan LiuLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Chenning ZhaoLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Dengliang WangInstitute of Fruit Tree Research, Quzhou Academy of Agriculture and Forestry Science , Quzhou 324000 ,Lili LiuInstitute of Fruit Tree Research, Quzhou Academy of Agriculture and Forestry Science , Quzhou 324000 ,Jinping CaoLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Yue WangLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,Chongde SunLaboratory of Fruit Quality Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University , Hangzhou 310058 ,

Horticulture Researchjournal2026en

ABI

Аннотация

Abstract Polymethoxyflavones (PMFs) are a distinct class of plant flavonoids with significant therapeutic potential, particularly as anti-cancer agents. This potent bioactivity is largely attributable to O-methylation. However, a major bottleneck for the industrial-scale production of PMFs is the lack of suitable precursors and available O-methyltransferases (OMTs), which are essential for constructing a viable biosynthetic pathway. Here, we discover a caffeic acid O-methyltransferase (CsOMT5) whose transcriptional dynamics closely mirrored the PMF levels in the flavedo of sweet orange (Citrus sinensis). In vivo validation further verified that CsOMT5 indeed promoted PMF accumulation in citrus. And its recombinant protein possessed broad substrate promiscuity for various PMF intermediates. Notably, CsOMT5 exhibited specialized C5-regioselectivity for naringenin, a readily available precursor for de novo synthesis of PMFs. Furthermore, critical residues involved this methylation activity (N14, T18, I120, I256, and G305) were revealed through mutational analysis. This study not only provides deeper insights into the PMF biosynthetic pathway but also opens new avenues for the industrial biosynthesis of PMFs.

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Темы

Bioactive Compounds in Plants Plant Gene Expression Analysis Plant Molecular Biology Research

Идентификаторы

DOI: 10.1093/hr/uhag128

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