ISOLATION OF THE KAEMPFEROL FLAVONOID FROM USMA PLANT USING CHROMATOGRAPHIC METHODS

This study focuses on the isolation of the flavonoid kaempferol from O‘sma plant material using chromatographic methods and evaluates the efficiency of the applied extraction and purification techniques. Flavonoids are an important class of plant secondary metabolites known for their significant biological activities, including antioxidant, anti-inflammatory, and anticancer effects. Among them, kaempferol is a widely studied flavonol with promising pharmacological potential. Therefore, the development of effective methods for its extraction and purification from natural sources is of considerable scientific and practical importance.In this research, the plant material was subjected to ethanol extraction, followed by liquid–liquid fractionation using solvents of increasing polarity to concentrate flavonoid compounds. The ethyl acetate fraction, identified as the most flavonoid-rich fraction, was further processed using silica gel column chromatography. Thin-layer chromatography (TLC) was employed to monitor the separation process and to identify fractions containing flavonoids, while high-performance liquid chromatography (HPLC) was used for the final confirmation of the isolated compound. The results demonstrated that the applied methodology allowed for the successful isolation of kaempferol with high purity, as confirmed by its characteristic retention time and chromatographic profile.

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