Статья

A 3347-Locus Genetic Recombination Map of Sequence-Tagged Sites Reveals Features of Genome Organization, Transmission and Evolution of Cotton (Gossypium)

Junkang RongPlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Colette A. AbbeyDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843John BowersPlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Curt L. BrubakerCommonwealth Scientific and Industrial Research Organization, Canberra, AustraliaCharlene ChangDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Peng W. CheeCoastal Plains Experiment Station, University of Georgia, Athens, Georgia 30602Terrye DelmonteDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Xiaoling DingDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Juan José Picazo de la GarzaDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Barry S. MarlerPlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Chan-hwa ParkPlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Gary J. PiercePlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Katy Martin RaineyPlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Vipin K. RastogiDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Stefan SchulzePlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Norma L. TrolinderDepartment of Plant and Soil Science, Texas Tech University, Lubbock, Texas 79409Jonathan F. WendelDepartment of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011Thea A. WilkinsDepartment of Agronomy and Range Science, University of California, Davis, California 95616T Dawn Williams-CoplinPlant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602Rod A. WingDepartment of Plant Sciences, University of Arizona, Tucson, Arizona 85721Robert WrightDepartment of Agronomy and Range Science, University of California, Davis, California 95616Xinping ZhaoDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Linghua ZhuDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843Andrew H. PatersonDepartment of Soil and Crop Science, Texas A&M University, College Station, Texas 77843

2004en

ABI

Аннотация

We report genetic maps for diploid (D) and tetraploid (AtDt) Gossypium genomes composed of sequence-tagged sites (STS) that foster structural, functional, and evolutionary genomic studies. The maps include, respectively, 2584 loci at 1.72-cM ( approximately 600 kb) intervals based on 2007 probes (AtDt) and 763 loci at 1.96-cM ( approximately 500 kb) intervals detected by 662 probes (D). Both diploid and tetraploid cottons exhibit negative crossover interference; i.e., double recombinants are unexpectedly abundant. We found no major structural changes between Dt and D chromosomes, but confirmed two reciprocal translocations between At chromosomes and several inversions. Concentrations of probes in corresponding regions of the various genomes may represent centromeres, while genome-specific concentrations may represent heterochromatin. Locus duplication patterns reveal all 13 expected homeologous chromosome sets and lend new support to the possibility that a more ancient polyploidization event may have predated the A-D divergence of 6-11 million years ago. Identification of SSRs within 312 RFLP sequences plus direct mapping of 124 SSRs and exploration for CAPS and SNPs illustrate the "portability" of these STS loci across populations and detection systems useful for marker-assisted improvement of the world's leading fiber crop. These data provide new insights into polyploid evolution and represent a foundation for assembly of a finished sequence of the cotton genome.

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Идентификаторы

DOI: 10.1534/genetics.166.1.389

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