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Linkage disequilibrium mapping of the bolting gene in sea beet using AFLP markers

Mats HansenNovartis Seeds AB, PO Box 302, S-261 23 Landskrona, Sweden. [email protected]Thomas KraftNovartis Seeds AB, PO Box 302, S-261 23 Landskrona, SwedenSARA GANESTAMNovartis Seeds AB, PO Box 302, S-261 23 Landskrona, SwedenTorbjörn SällDepartment of Genetics, Lund University, Sölvegatan 29, S-223 62 Lund, SwedenNils‐Otto NilssonNovartis Seeds AB, PO Box 302, S-261 23 Landskrona, Sweden
2001en
ABI

Аннотация

The possibility of using linkage disequilibrium mapping in natural plant populations was assessed. In studying linkage disequilibrium among 137 mapped AFLP markers in four populations of sea beet (Beta vulgaris ssp. maritima (L.) Arcang.) it was shown that tightly linked loci could be detected by screening for associations. It was hypothesized that the short distances spanned by linkage disequilibrium enable markers that are very tightly linked to a target gene to be identified. The hypothesis was tested by whole-genome screening of AFLP markers for association with the gene for the annual growth habit, the B gene, in a sample of 106 sea beets. Despite the dominant nature of AFLP, two markers showing significant linkage disequilibrium with the B gene were detected. The results indicate the potential use of linkage disequilibrium for gene mapping in natural plant populations.

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